Jennifer  and Rene,
 
As Rene said the
   NaCl  is  part  of the PBS. The 0.9% concentra= tion is equal to
   0.15  M.  I have heard (but never tested) of somebody = who raised
   the  NaCl  concentration  to  0.5  M  for  reducing non-specific ba   ckground  staining. He used this high salt PBS not only for diluting
   the=  primaries but also as washing buffer. Take care this high salt
   concent=  ration  only  works  for  FFPE  sections. It will
   certainly     destroy=     your     acetone-fixed    cryo's!
   
Cheers,
      
Chris     van     der     Loos,
   PhD
Dept.   of  Pathology
Acade=  mic  Medical  Center
   M2-230
Meibergdreef  9
NL-1105 AZ Amsterd= am
The
   Netherlands
 
 
 
Date: Tue, 27
   Nov   2007  09:44:33  -0800  (PST)
From:=  Rene  J  Buesa
   <rjbuesa@yahoo.com>
Subject:         Re:   [Histonet]  NaCl  in  antibody  diluent
To:  "Harvey,
   Jennif= er Lynn"
   <jennifer.harvey@Vanderbilt.Edu>,
   histonet@lists.utsouthwestern.edu

Jennifer:=     The Ab diluent is made of 1% bovine serum albumen
   in  PBS  wit= h ).05% Tween 20 and 0.1% sodium azide.'NaCl
   is  part  of  the  P=  BS but I have never used or kbown anybody using
   additional NaCl
&= nbsp; René
   J.

"Harvey,     Jennifer     Lynn"    <   jennifer.harvey@Vanderbilt.Edu>
   wrote:
    H=  istonet,

Has  anyone
   tried   NaCl   in   there   diluent   buffer   f=   or  IHC,  if  so
   what
concentration.   I   read   about   it   in  DAKO's     immunostaining  methods  booklet
but  they  don't  state  the
   concentr=  ation.  This  is my last chance with my
background
   problem.   I  ha=  ve  a  sticky  collagen  issue  I  cant  get  rid
   of.

Thanks!<= BR>

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