[Histonet] Problems in Neurofilaments-31 Staining


Hi guys,
We are right now working on Neurofilaments SMI-31 staining from Abcam 
laboratory on brain tissues. We had used 1:100, 1:500, 1:1000 dilutions of 
that stain. But still, it is very light. We had also did pre-treatment 
with citrate buffer and also with TBS buffer. Can anyone give suggestions 
about this staining? Do you have also some problem with this antibody or 
from same company?

Daivik Shah, Histotechnologist, M.S. Biotechnology
RADC Laboratory, Cohn Building 436
Rush University Medical Center

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