Our lab is demoing a Dako immuonstainer and I am having a hard time
optimizing our Melan-A. The dilutions I have tried are 1:50, 1:100, and
1:200. I have tried antigen retreival with EDTA pH9 and Citrate buffer
at pH 6. I am incubating in the primary and polymer for 20 minutes
each. I am still having a lot of trouble with excessive muddy
background. Any ideas??
Thanks,
Joannah Miley, HT(ASCP)
NW Pathology
Bellingham, WA
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|