[Histonet] Melan-A on Dako Immuno Stainer

From:"Miley, Joannah C."

Our lab is demoing a Dako immuonstainer and I am having a hard time
optimizing our Melan-A.  The dilutions I have tried are 1:50, 1:100, and
1:200.  I have tried antigen retreival with EDTA pH9 and Citrate buffer
at pH 6.  I am incubating in the primary and polymer for 20 minutes
each.  I am still having a lot of trouble with excessive muddy
background.  Any ideas??
Joannah Miley, HT(ASCP)
NW Pathology
Bellingham, WA
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