[Histonet] Frozens from Lung tissue

From:"PETER CRAVEN"



Gudrun 

We used to embed our lung blocks in Gelatin prior to freezing in OCT to
help hold the lung together when frozen sectioned

Rinse out the formalin

10% Gelatin till the block of tissue sinks

20% Gelatin overnight

Change to fresh 20% and set in a fridge to set the gelatin

Trim off the excess gelatin

Adhere to cryostat block with OCT and cut

Hope this helps

Peter

 

 

Peter L Craven FIBMS

BMS 2

Pathology Department

Raigmore Hospital

Inverness IV2 3UJ

tel. 01463 704000 ext. 4269

email: peter.craven@haht.scot.nhs.uk 

 


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you have received this email in error please notify
the system manager.
This footnote also confirms that this email message has been swept by
MIMEsweeper for the presence of computer viruses.
www.mimesweeper.com
**********************************************************************
Please consider the environment before printing this e-mail
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>