Gudrun
We used to embed our lung blocks in Gelatin prior to freezing in OCT to
help hold the lung together when frozen sectioned
Rinse out the formalin
10% Gelatin till the block of tissue sinks
20% Gelatin overnight
Change to fresh 20% and set in a fridge to set the gelatin
Trim off the excess gelatin
Adhere to cryostat block with OCT and cut
Hope this helps
Peter
Peter L Craven FIBMS
BMS 2
Pathology Department
Raigmore Hospital
Inverness IV2 3UJ
tel. 01463 704000 ext. 4269
email: peter.craven@haht.scot.nhs.uk
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