Sure, you are right. I hoped to go a step up on the ladder of quality with
some small changes in our method, because we are not familiar with the real
snap-freezing. For all other tissues the simple method is good enough, just
the lung makes a headache. And this special situation only occurs once in
two months and only with our primarius (head of institute).
Von: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk]
Gesendet: Freitag, 23. November 2007 16:24
Betreff: RE: [Histonet] frozens from lung-tissue
My original question was, how to get the best frozen lung-slides without
the use of liquid nitrogen or isopentan. - just with the cryocut, chucks
and OCT. The pathologist complains about "overfrozen" edges and a bad
overall morphology. I will ask him for more descriptionary details or
get a picture. Thank you for your hints.
But that's the thing I'm struggling with. Liquid nitrogen with or
without 'additives' is the best way of reducing ice artefact, so by not
using it I expect you to get worse results. If you want 'better'
morphology then post fix.
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