You don't say what detection system you are using....However, if  you are 
using a Biotin-avidin/streptavidin detection, the background  may be due to the 
exposure of endogenous biotin by HIER.  You may need  to incorporate an 
appropriate blocking agent/system  
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital





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