Re: [Histonet] Short processing schedule-suggestions

From:"Anthony Reilly"

Hi Greg

We do a lot of short cycling of biopsies and until recently were using
an older model VIP circa 1990. I found that a 2 hour cycle was adequate
for your  standard sized gut biopsies, however longer was required for
the polypoid lesions which can come in all shapes and sizes.

So in short  for standard sized biopsies (up to 4mm diameter) 2 hours
is adequate but if you wanted to be conservative 3 or 4 hours would not
be too long.


Tony Reilly
Chief Scientist
Anatomical Pathology
Pathology Queensland
Prince Charles Hospital
Rode Rd Chermside Q 4032
Ph: 07 3139 4543
Fax: 07 3193 4546

>>> "Greg Dobbin"  11/09/07 12:59 am >>>
Hello All,
I would like to get your suggestions for a processing schedule for
small biopsies (cores, GI's, other endoscopics). I want to process
little guys separate from everything else to hopefully improve section
quality and perhaps staining quality. 

I don't want the absolute shortest possible schedule. We have a newer
VIP5 for our main processor (doing everything on one schedule
and a much older VIP sitting idle as a backup that I plan to start
for the biopsies. We  process overnight so I have more than enough
I will just delay the biopsy processing so that both processors are
finished at essentially the same time. 

What I need is a shorter schedule for the biopsies so that the
detrimental affects of xylene and alcohol are minimized (and yes, we
still use xylene! And in case it matters schedule-wise, we use 10%
as well).

Thanks in advance.

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

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