Re: [Histonet] Short processing schedule-suggestions

From:Rene J Buesa

>From my experience in processing, the most important thing is to maintain dehydration time to clearing time to infiltration time in constant proportions once you find a good protocol.
  If you "long" protocol works well for you, reduce the time in each step in a way that at the end will be completed in the time you need, BUT preserving the time proportions you have now.
  René J.

Greg Dobbin  wrote:
  Hello All,
I would like to get your suggestions for a processing schedule for
small biopsies (cores, GI's, other endoscopics). I want to process these
little guys separate from everything else to hopefully improve section
quality and perhaps staining quality. 

I don't want the absolute shortest possible schedule. We have a newer
VIP5 for our main processor (doing everything on one schedule currently)
and a much older VIP sitting idle as a backup that I plan to start using
for the biopsies. We process overnight so I have more than enough time.
I will just delay the biopsy processing so that both processors are
finished at essentially the same time. 

What I need is a shorter schedule for the biopsies so that the
detrimental affects of xylene and alcohol are minimized (and yes, we
still use xylene! And in case it matters schedule-wise, we use 10% NBFS
as well).

Thanks in advance.

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

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