Probably because we tend to shake in/out quickier when staining manually that the stainer does. It takes to the stainer more time to take slides out-transport to the next station- and into the next station than we to us manually. That extra time can account for the difference.
  René J.

karenadams@comcast.net wrote:
  
We are presently using Gill's III manually for H & E and on the automated stainer.....I am seeing mucin lightly staining on the automated stainer, but not the manual.....any thoughts??
--
Karen Adams 
Supervisor
Pathology Laboratories West 
9303 Park West Blvd 
Knoxville, TN 37923 
(865) 690-2111 FAX (865) 691-1623
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