RE: [Histonet] processing

From:"Tarango, Mark"

Can you use 20% formalin on breast tissue?

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
[] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, November 13, 2007 10:36 AM
Subject: [Histonet] processing

Now that we can no longer use alcoholic fixatives on breast tissue, is
there anyone out there that is putting a slight temperature on the
reagents on the processor to enhance fixation and dehydration?  I have
thought of using stronger alcohol grades and an extra xylene, which
would help in the dehydration of the lipids.  This would be a dedeicated
processor for fatty tissue types only.  What is everyone's thoughts and
thanks for all input!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
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