RE: [Histonet] deplasticizing PMMA sections for staining

From:"Michelle McDonald"

We actually use Acetone to deplasticise MMA sections. Only takes 2
changes for 10 mins each.


Dr Michelle McDonald B.MedSci, PhD
Research Officer
Orthopaedic Research Dept.
The Children's Hospital Westmead.
Westmead NSW 2145
Ph. +612 9845 1451
Fax. +612 9845 3078

-----Original Message-----
[] On Behalf Of Monfils,
Sent: Tuesday, 6 November 2007 6:22 AM
Subject: RE: [Histonet] deplasticizing PMMA sections for staining

In my experience, xylene is a rather poor solvent for PMMA.  It works
much faster at 60 degrees, but don't try heating flammable solvents in
your oven unless you are certain it is certified explosion-proof (most
paraffin ovens are not).  Even then, unless you have the oven in or at
least next to a fume hood, you'll get a lot of xylene vapors in the air
as a result of heating it.  I deplasticise PMMA with 2-methoxyethyl
acetate, which only takes a couple of hours at room temperature.  This
has a rather strong though not unpleasant odor, and should be used in
the hood.

Histonet mailing list

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

Histonet mailing list

<< Previous Message | Next Message >>