RE: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens

From:"Douglas D Deltour"


We also are having the same issue with the Surgipath Gills III. This issue
also started last month. Very interesting. 

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262 
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
[] On Behalf Of
Sent: Monday, November 05, 2007 4:09 PM
To: DNon;
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens

I find this post most interesting.  After using Surgipath Gills III for
the past 7 years we started having a problem with blue mucin just last
month.  I corrected the problem by adding GAA but I now have to monitor
the pH on a daily basis.  It is strange that after all these years I
would suddenly have this problem and then read about another lab having
the same difficulty with Surgipath Hematoxylin starting about the same
timeframe.  Has Surgipath changed something?

Charles Embrey Jr., PA(ASCP)
Histology Manager, Carle Clinic 

-----Original Message-----
[] On Behalf Of DNon
Sent: Saturday, November 03, 2007 9:42 AM
Subject: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens

Hello Histonet, 

     Over the past several weeks our lab has had problems with
hematoxylin overstaining in the mucin of our gastric specimens.  The
degree of hematoxylin overstaining is alarming in the mucin of the
gastric specimens but absent or negligible in other specimens.  One
pathologist reviewing the slides indicated some of the overstained
specimens appeared to be inadequately fixed.  We have addressed that
issue which showed some improvement, but the problem persists.  We are
making changes to increase fixation time (although the specimens now
appear adequately fixed yet retain a large measure of the overstaining),
but any further suggestions for corrective action would be much

    We've been hesitant to increase acid alcohol clearing time because
our other specimens are staining well with the current protocol.

We are using the following reagents and stain times:

-  Surgipath specimen containers pre-filled with 10% neutral buffered
-  Surgipath Gills III Hematoxlyin - 6.5 minutes followed by running
water washes
-  .75% HCL in 70% ETOH for 1 second (1 dip) followed by running water
-  Surgipath Scott's Tap Water for 1 min followed by running water wash

Sakura automated stainer set to agitate ( up and down dipping of racks )
once every 2 seconds, which is the fastest speed available on it.  

Dick Non
Pathology Department
Ocean County Medical Lab
Brick, New Jersey
Histonet mailing list

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>