RE: [Histonet] Matrigel as culture medium

From:"Keller, Charles"

Dear Teri, a possible solution is to use Extracel (from Glycosan Inc in
Utah) instead of Matrigel.  I've fixed Extracel in 10% formalin and
sectioned in in paraffin.  It has the advantage of being synthetic
(Matrigel has viral contamination from time to time).  Sincerely,

-----Original Message-----
[] On Behalf Of Johnson,
Sent: Friday, November 16, 2007 9:57 AM
Subject: [Histonet] Matrigel as culture medium

One of our researchers is using matrigel as a culture medium on
chambered slides. We tried paraffin embedding them, and found the
Matrigel wasn't really solid enough to hold the cellular structure in
place. We found it particularly difficult to keep some sort of
consistency scraping it off (it had the consistency of warm jelly).

I'd love to hear some ideas on how we can accomplish sample processing
without centrifugation. Ideally it would be good to encase the cells in
a matrix that would solidfy enough to embed as a cell block, keeping the
cellular structure intact. 

Thanks for your help!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

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