RE: [Histonet] Giemsa Stain

From:"Tarango, Mark"

Shit!  I can't get my giemsas to stain!  

Seriously, if you're looking for H. Pylori, you should have an H. Pylori

If you are doing a giemsa on a blood smear, then you should have WBCs
there to stain all those pretty colors (in which I can't see why you'd
need an H. Pylori control).  It depends on the reason you're doing the

Looking for H. Pylori this way seems old school anyway.  I'd rather go
to a hospital that checks by IHC or alcian yellow. I like what I'm
staining to stand out.

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
[] On Behalf Of Marshall
Terry Dr, Consultant Histopathologist
Sent: Wednesday, November 14, 2007 5:51 AM
To: Kemlo Rogerson; Rene J Buesa; Schaundra Walton; Histonet
Subject: RE: [Histonet] Giemsa Stain

The tissue can still be blue even if the organism isn't there.

BTW, has anyone ever seen a Giemsa that doesn't stain? I can't really
imagine it.


-----Original Message-----
From: Kemlo Rogerson [] 
Sent: 14 November 2007 13:42
To: Marshall Terry Dr, Consultant Histopathologist; Rene J Buesa;
Schaundra Walton; Histonet
Subject: RE: [Histonet] Giemsa Stain

"If the tissue is blue, it has worked, at least to the extent it needs
to have done for this purpose."

If it's not blue then it hasn't worked or the organism isn't there!

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown 

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