RE: [Histonet] B5 substitute

From:Rene J Buesa

Amen! Only make absolutely sure that the pH of your NBF is neutral (7).
René J.

"Tarango, Mark"  wrote:  Why not just use 10% NBF? It's great if you allow enough time for
fixation. Make sure your bone marrows are fixed well BEFORE
decalcifying and you should have no problems.

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
[] On Behalf Of Joanne
Sent: Thursday, November 08, 2007 8:51 AM
Subject: [Histonet] B5 substitute

Hi ,

What are you using to replace B5 fixative with mercury? I want the best
for hematopoetic tissues, and for immunostains.


Jo Mauger

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