An antibody vendor recommended pepsin pretreatment prior to primary
antibody incubation of our free-floating 40 um formaldehyde-fixed rat
brain frozen sections, but provided a protocol that only listed pepsin
by weight. Since enzyme activity ranges from 3 to 4500 units/mg in
commercial sources, does anyone have a rough idea what kind of activity
is sufficient without destroying the tissue (which is what happened with
the first test). Anybody find this approach worthwhile with similar tissue?
Thanks,
Mike King
UF Pharmacology & Therapeutics
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