[Histonet] in situ and immunohistochemistry

From:Jerry Siegel

I would like combine in situ and immunohistochemistry on the same tissue, 
double labelling for mRNA and cell protein.  I notice that published 
protocols use fixed tissue for the initial in situ step followed by 
immuno.  We would ultimately like to perform this procedure on human tissue 
that has been in fix for years.  We have been able to do immuno on this 
tissue using antigen retrieval techniques.  Does anyone have any experience 
doing in situ on tissue that has been in fix for years?   Does anyone 
recommend particular protocols for this double labelling procedure?

J. Siegel 

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