[Histonet] how to keep thick sections on the slides after deparaffin


  I met a big problem in keeping thick sections on the slides after
deparaffin recently. I cut a 70um-thick mouse retina section on
superfrost plus Gold slides, leaving it dry for 2days. Before
deparaffin, I put the slide in the 65C oven for 30 min to let it melt,
then I did the general deparaffin procedure. Unfortunately, the section
fell off from the slide. Does anyone have any experience on deparaffin
thick sections? Any suggestions?
  Thank you,
Ann Dongtao Fu MD Ph.D
Lab Manager
Molecular Pathology core
Dept. of Pathology
University of Florida
Lab Phone: 352-273-7752
FAX: 352-273-7753
Rm: D11-50
Histonet mailing list

<< Previous Message | Next Message >>