hi histonetters:
the PCNA protocol listed on ihcworld (http://www.ihcworld.com/_protocols/antibody_protocols/pcna_santa_cruz.htm) doesn't mention what secondary antibody to use when doing PCNA staining. does anyone know the recommended secondary? also, does anyone have a better/different protocol for PCNA? I'm using Calbiochem's mouse anti-PCNA. Thanks for the help.
William Ares
Research Technician
Laboratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University
Tel: (212) 746 5056
Email: wia2005@med.cornell.edu
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