It sounds as though you are processing your tissues too long, and too much
water is being removed (over dehydration.) In general, a properly
processed mouse tissue after a short schedule will need a bit of a water
soak, but not for 6 hours. Another trick is try room temperature or even
warm water for a few minutes, then go to ice water, and be careful to NOT
cut away what you have soaked - be careful to reapproach the blade in order
to cut the sections. Some people have suggested floating blocks face down
on a waterbath for a very short time.
if you post your processing schedule, Histonetters can help solve some of
----- Original Message -----
From: "Eva C Andersson"
Sent: Tuesday, November 13, 2007 2:32 PM
Subject: [Histonet] microtomy technical question
> Hello everyone,
> I have some questions regarding microtomy.
> We place our blocks face down on ice. The problem is that some tissues
> take a very long time before they are ready to be cut(some more than 6
> hours). For some tissues like kidney samples (mouse tissue) we have been
> using glycerol on the ice. This does seem to cut down on the time needed.
> My question is which other tissues can I use this technique on? Do you
> have any other suggestions for how to get the tissue hydrated for cutting?
> Thank you for your help,
> Eva Permaul
> Georgetown University
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