Re: [Histonet] Hexane freezing Method for Cryosectioning
From: | "Markus F. Meyenhofer" |
Why solvents? To counter the "Leidenfrost Effect" . Google or wikipedia for
the explanation>
Markus
----- Original Message -----
From: "patsy ruegg"
To: ;
Sent: Friday, November 24, 2006 4:25 PM
Subject: RE: [Histonet] Hexane freezing Method for Cryosectioning
> Pam,
> Hexane is still very flammable, not sure if it is any less explosive, both
> would probably be considered pretty hazardous. If you are using liquid
> nitrogen why do you also need the solvent? I snap freeze by putting oct
> around the sample in a cryo mold, then slowly lower into liquid nitrogen
> with good results for frozen sections on undecalcified bone, better yet
> for
> tape transfer sections, I fix, wash and infiltrate over night at 4dc in
> 30%
> sucrose, then do the snap freezing with OCT and liquid nitrogen. John
> Trapley wrote an article for JOH on this several years ago.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> pmarcum@vet.upenn.edu
> Sent: Friday, November 24, 2006 11:49 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Hexane freezing Method for Cryosectioning
>
> Good Afternoon,
>
> I have been asked to look into using hexane in rapid freezing of tissues
> (primarily non-decalcified bone). I am looking it up with several other
> searches and wondered if anyone is currently using this technique. Is it
> better or safer than isopentane with liquid nitrogen? I know the issues
> with
> isopentane and explosions so this partly a safety questions also.
>
> Thanks for the help in advance.
>
> Pam Marcum
> UPENN Vet School
> New Bolton Center
>
>
>
>
>
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