Re: [Histonet] Anti GFP on cryosections

From:"Gregory A. O'Sullivan"

Dear Adi

It is not clear if you are using transgenic mice expressing GFP and if you
are able to see GFP fluorescence
from these frozen cryosections. However, in our experience with Transgenic
mouse lines expressing GFP
that have been frozen prior to crosection preparation, is that we loose GFP
fluorescence and are unable
to detect GFP with a primary antibody. However, if we fix the tissue in 4%
PFA and subsequently
cryoprotect with 30% Sucrose prior to the preparation of crysections, we can
see nice GFP fluorescence.
So if you just want see whether GFP is being expressed, then I would try
this first. The primary antibody
we used is the one from Clontech.



----- Original Message ----- 
From: "Adi Sabag" 
To: "Histonet" 
Sent: Wednesday, November 29, 2006 11:40 AM
Subject: [Histonet] Anti GFP on cryosections

Hi All,

Does anyone have an experience with anti-GFP antibody of MBL on



Adi Sabag
Tumor Progression and Angiogenesis laboratory
Medicine Faculty, Technion
Haifa, Israel.

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