RE: [Histonet] weils stain for myelin

From:"Gidmark, Nicholas"

I've used Erlich's haematoxylin and my recipe has 0.5 grams of sodium iodate as a good instant-ripener for 600ml of solution, just to give you an idea of scale.
Good luck!
Nicholas J. Gidmark, graduate student
Brainerd lab of biomechanics and functional morphology
Dept. of Ecology & Evolutionary Biology
Brown University
80 Waterman Street, box G-W
Providence, RI 02912
Cell: 612.386.0042
Lab: 401.863.1032


From: on behalf of stevenk
Sent: Tue 28-Nov-06 11:39 PM
Subject: [Histonet] weils stain for myelin

        I am asking for a colleague who was doing a Weil's stain for myelin.

        One of the ingredients, 2% alcoholic haematoxylin requires a
ripening process for at least 6 months.

        As the student cannot wait that long, I was wondering if
anyone could supply a quicker method, or suggest something to hasten
the ripening process of the above.

        Thanks from down under

|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Ph: + 61 2 9351 6143
|Fax:+61 2 9351 3429

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