RE: [Histonet] OCT questions

From:Rene J Buesa

Dear Surita:
  As standard procedure for direct immunofluorescence I always used distilled water to do skin frozen sections.
  The cryostat has to be at least at -20║C and you addd a large drop, place the specimen inside it and keep building more ice around it as it freezes.
  Nothing to wash afterwards!
  RenÚ J.

Alan Bright  wrote:
  Dear Surita,

Yes use water to attach your tissue, this was the main method used when
we first started manufacturing cryostats in the 50's.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
Skype User ID: dazzle0


-----Original Message-----
From: Surita Banwait [mailto:sbanwait@buckinstitute.org] 
Sent: 22 November 2006 18:14
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OCT questions





Hi There,

Would anyone happen to know if there is a way to attach frozen tissue to
cryostat chucks without using OCT media or super glue? 



Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media?



Thanks,

Surita


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