[Histonet] Re: Fixation and Immunos
Kimberly Secrest HTL, QIHC at West Virginia University Hospital asks about >>
using alcohol/formalin combination fixatives [such as] Penn-fix has been
brought up by pathologists and [pathologist's assistants], but there is also
concern about the effects the fixative may have on immunostaining.<<
You'd be using a fixative like that mostly to find small lymph nodes in colon
resection specimens and some other cancer resection specimens (not including
breast, at least for my personal preference). You wouldn't being doing
immunostains on such material anyway. If you need to do them in the future, you'll
just have to try and see.
I prefer making up my own fixative (such as Davidson's fixative, which is
three parts water, three parts alcohol, two parts strong [37%] formalin, one part
glacial acetic acid) or using a commercial product called O-Fix.
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