[Histonet] RE: Alizarin red S - Calcium
The pH or Alizarin Red is very critical so I would try and keep it as close to the recommended pH as possible. Also, be careful when you wash the section/cells with PBS or something as this really mucks it up... may be obvious but I found out the hard way!!
Stem Cell Imaging
MRC Clinical Sciences Centre
Imperial College London
Hammersmith Hospital Campus
Du Cane Road
Date: Mon, 20 Nov 2006 10:14:31 -0500
From: Diego Villegas
Subject: [Histonet] Alizarin red S - Calcium
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The protocol for Alizarin red S to identify calcium suggest a solution
pH 4.1 to 4.3. The Alizarin solution of our provider has a 4.5 to 5
pH. Could I use it, or is critical the pH?
Michigan Technological University.
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