Re: [Histonet] pH of EDTA
We use in-house EDTA buffer for retrieving some antigens. We have noticed,
that the pH decreases as the buffer gets older. We make a litre at a time
and use it within the week. It seems this buffer is not stable and maybe pH
needs to be checked and adjusted each time you use it. Maybe you can adjust
the pH after diluting it for use, with very dilute sodium hydroxide.
I find EDTA buffer bothersome, if your detection system is avidin based,
since it brings out the endogenenous biotin in many tissues. We end up doing
a biotin block on all the EDTA retrieved tissues.
Hamilton, Ontario, Canada
----- Original Message -----
From: "donna rossi"
Sent: Thursday, November 02, 2006 5:50 AM
Subject: [Histonet] pH of EDTA
> We are having a problem with EDTA pH 8.0 from LabVision. When we take
> the pH of the concentrate it is 8.O. When we dilute it as recommended
> it changes to 6.0. Lab Vision replaced our bottles but the new
> bottles also come out to 6.0 when diluted. We took the pH of the
> Distilled water here and got 7.1. What could be the problem.? Do we
> adjust the distilled water to a higher pH? How do we do that? Do we
> then need to validate all of our antibodies that use EDTA using the"
> new" EDTA? We have also had this problem with Zymed EDTA but not as
> radical a difference as the Labvision. It was suggested that we
> switch to ready-made EDTA but we have a quantity of this and do not
> have the room to store multiple big bottles of this retrieval buffer.
> Can someone give me a suggestion? Thanks, Donna, SRHS
> Use your PC to make calls at very low rates
> 1. http://g.msn.com/8HMAENUS/2749??PS=47575
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