Send a detailed account of your technique. 
You will many very helpful replies.
__________________ 

sohail ejaz wrote:
> 
> I am studying tracheal vasculature by using vWF as blood vessel marker.
>   After staining i am using toluene to clear the tissue, but after using that flouresence goes down..................??
> 
>   Can anyone guide me for to slove this issue.
> 
>   If some one have a better protocol for tracheal whole mounnt staining, I would love to try for that.
> 
>   Regard
> 
>   Dr. Rudiger Tom
>   University of Connecticut, Storrs
>   Connecticut
> 
> 
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