Re: [Histonet] Thin Paraffin Sections
0.5 um is very thin for paraffin medium. The thinnest paraffin section we
have cut is 1 um using Accuedge disposable blades, either high or low
profile. When we need the thinner 0.5 um sections, we use glycol
methacrylate plastic embedding and section with glass knives. If you
decide to cut thin paraffin, a harder paraffin often is better - there are
several on the market.
At 10:18 AM 11/6/2006, you wrote:
>Someone came by our lab and asked about very thin sections in paraffin,
>0.5 um to be exact.
>Has anyone any experience or information on this.
>Thank you very much.
>Lin S. Bustamante, B.Sc.; HT(ASCP)
>Dept. of Veterinary Anatomy and Public Health
>Texas A&M University
>College Station, TX 77843-4458
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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