We created nuclear bubble artifact by flattening a just picked up section 
(from waterbath) on a really hot surface or hot plate. One  person just 
addressed this today by saying too hot a waterbath contributes to this 
also.  We quit flattening the section on a hot plate, and simply drained 
the slide, then went to a lower heat incubator - 60C is probably adequate 
for a short time or until paraffin is melted.

At 11:14 AM 11/8/2006, you wrote:
>I know this subject has come up before, and I may have missed it on Histonet,
>but...
>
>How do you trouble shoot nuclear bubble artifact?
>
>Nuclear bubble artifact really degrades pathologic diagnosis. I've never been
>clear how to advise a histotechnologist on how to look for the cause and fix
>it. Can some of the senior histotechnologists on HIstonet address this issue
>for all of us?
>
>Bob Richmond
>Samurai Pathologist
>Knoxville TN
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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