Re: [Histonet] Help with immunohistochemistry in human embryos
How do I remove myself from this list? I have e-mailed histonet.com
but it hasn't worked? Any help is appreciated.
Thank you
______________________________________________________________
From: "Amy Porter"
To: "Hernan Aldana"
,
Subject: Re: [Histonet] Help with immunohistochemistry in human
embryos
Date: Fri, 10 Nov 2006 10:24:11 -0500
>You might want to try using a Heat Epitope Induced Retrieval
>protocol. Most of Santa Cruz's antibodies will work with heat
>retrieval.
>Amy S. Porter, HT (ASCP) QIHC
>Investigative HistoPathology Laboratory - Supervisor
>2201 Biomedical Physical Sciences Bldg. Rm #2133
>East Lansing, MI 48824-3320
>Phone: (517) 355-6475 ext 1480
>Fax: (517) 432-1368
>Email: portera@msu.edu
>Web: www.humanpathology.msu.edu
>----- Original Message ----- From: "Hernan Aldana"
>
>To:
>Sent: Thursday, November 09, 2006 10:45 AM
>Subject: [Histonet] Help with immunohistochemistry in human
embryos
>
>
>Dear Histoneters
>I need some advices. I'm working with human embryos between 7 and
14
>weeks.
>we used Sonic Hedgehog Shh (C-18) and sc-1195 antibodies of Santa
>Cruz
>Biotechnology in paraffin sections of embryos fixed in
>paraformadehyde. I
>don't have good results.
>Could anybody help me with some advice, concerning to every step
of
>my
>technique?
>
>Thanks in advance
>
>Dr. Hernán Javier Aldana Marcos
>
>Prof. Titular Reg. Histología, Embriología y Biología Celular
>
>
>Facultad de Medicina
>Universidad de Morón
>Argentina
>
>email altenrativo: hernanjavier@yahoo.com
>http://www.ht.org.ar/educacion.htm
>
>
>
>
>
>-----Mensaje original-----
>De: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] En nombre de
>histonet-request@lists.utsouthwestern.edu
>Enviado el: Domingo, 01 de Octubre de 2006 02:04 p.m.
>Para: histonet@lists.utsouthwestern.edu
>Asunto: Histonet Digest, Vol 35, Issue 1
>
>Send Histonet mailing list submissions to
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>When replying, please edit your Subject line so it is more
specific
>than "Re: Contents of Histonet digest..."
>
>
>Today's Topics:
>
> 1. Job Opening (Luck, Greg D.)
> 2. RE: Problem of Double immunoflouresence staining ..
> (Melissa Gonzalez)
> 3. The procedure that substitues xylene with mineral oil.
> (PhiHo Hoang)
> 4. Re: The procedure that substitues xylene with mineral oil.
> (Rene J Buesa)
>
>
>------------------------------------------------------------------
----
>
>Message: 1
>Date: Sat, 30 Sep 2006 12:01:02 -0700
>From: "Luck, Greg D."
>Subject: [Histonet] Job Opening
>To:
>Cc: "Ringwood, James F." ,
"Wakabayashi,
>Marlene T." , "Browning, Rachel M."
>
>Message-ID:
><6BB8BC4519AAB844B174FC739A679BBC50900C@IRMEXCH01.irm.inhs.org>
>Content-Type: text/plain; charset="us-ascii"
>
>Hello All,
>
>Deaconess is a 300 bed acute care community medical center. We
have
>a
>full time, M-F, day time position open. Must be HT(ASCP)
certified
>or
>eligible. Duties include all routine histology functions in a
>clinical
>setting (i.e. embedding , cutting, special stains and frozen
>sections).
>IHC experience preferred. Due to a recent market adjustment by
our
>organization the salary range for this position has just increased
>25-33% depending on years of experience. This is a golden
>opportunity
>for someone to improve on their current situation or to begin
anew.
>Anyone new to this field is encouraged to apply as well, for the
>door is
>wide open. You can apply on-line by going to our corporate
website
>www.empirehealth.org. The position is at Deaconess Medical Center
>in
>Spokane, WA www.deaconess-spokane.org. Please contact me directly
>for
>more details. Following is an additional web link to provide you
>with
>more info on Spokane and the greater Inland Northwest region
>www.visitspokane.com. ("Near Perfect-Near Nature"). Thank you and
>best
>wishes, Greg
>
>Greg Luck, BS, HT(ASCP)
>Anatomic Pathology Supervisor
>Deaconess Medical Center
>800 W. 5th Ave
>Spokane, WA 99204
>Phone 509.473.7077
>Fax 509.473.7133
>luckg@empirehealth.org
>www.deaconess-spokane.org
>
>
>
>------------------------------
>
>Message: 2
>Date: Sun, 1 Oct 2006 08:40:44 -0700
>From: "Melissa Gonzalez"
>Subject: [Histonet] RE: Problem of Double immunoflouresence
staining
>..
>To:
>Message-ID:
>
>Content-Type: text/plain; charset="us-ascii"
>
>Sohail,
>First, Perhaps you should try incubating each primary with
>respective
>secondary only, to determine how they look separately, and also
make
>sure each is working on its own. For example, you may not have
your
>aSMA
>dilution correct. Also, do you have a positive control for TRITC
to
>make
>sure you can detect any TRITC signal adequately? Once you have
>appropriate signal from both, you can then make your cocktails.
>Also, in
>the future, I would recommend switching to Alexa Fluors from
>Molecular
>Probes, they really are great. In your instance you would use
AF488
>(FITC) and AF 546 or 555 (TRITC). But that is just my 2 cents.
>
>Good luck,
>Melissa
>
>
>
>Message: 4
>Date: Fri, 29 Sep 2006 13:06:27 -0700 (PDT)
>From: sohail ejaz
>Subject: [Histonet] Problem of Double immunoflouresence staining
of
>retinal vasculature
>To: histonet@lists.utsouthwestern.edu
>Message-ID: <20060929200627.949.qmail@web39506.mail.mud.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>Hello everybody
>
> I have a big problem of staining retina vasculature using a
>cocktail
>of two different antibodies. The problem is that i can only see
>staining
>with FITC but not with TRITC, hence i cant make a merger of both
>FITC
>and TRITC.
>
> Cocktail of primary antibodies include (1) Monoclonal Rabbit
Anti
>human vWF (2) Monoclonal mouse Anti alpha SMA and cocktail of
>secondary
>antibodies include (1) Anti rabbit FITC (2) Anti mouse TRITC
>
> Please let me know your commentc to slove this issue.
>
> Dr.Sohail
>
>
>
>
>
>------------------------------
>
>Message: 3
>Date: Sun, 1 Oct 2006 11:47:56 -0400
>From: "PhiHo Hoang"
>Subject: [Histonet] The procedure that substitues xylene with
>mineral
>oil.
>To:
>Message-ID: <005b01c6e570$f725b820$f300a8c0@ttth>
>Content-Type: text/plain; charset="iso-8859-1"
>
>
>Greetings,
>
>I am a new subscriber to this list and I am reading
>the list archive.
>
>I came across a posting from Reni J. responding to
>a request from Sharon:
>
>
>
>Sharon:
> I am sending privately a procedure I have that substitutes
xylene
>with
>mineral oil. It is 100 times less toxic than xylene and 3 times
>cheaper.
> Reni J.
>
>Sharon Allen exchange.hsc.mb.ca> wrote:
>
>Does anyone use xylol substitutes, if so how good are they?
>We do CJD testing & "the powers that be" don't like to pay for
>getting rid
>of contaminated xylol. I don't want to have to start testing
>different
>substitutes, so any help would be greatly appreciated.
>Thanks
>Sharon
>sallen <@t> hsc.mb.ca
>
>
>
>I am interested in this procedure.
>
>It is very much appreciated if I can get a copy
>of that procedure.
>
>Best regards,
>
>PhiHo
>
>
>
>
>
>------------------------------
>
>Message: 4
>Date: Sun, 1 Oct 2006 09:55:53 -0700 (PDT)
>From: Rene J Buesa
>Subject: Re: [Histonet] The procedure that substitues xylene with
>mineral oil.
>To: PhiHo Hoang ,
>histonet@lists.utsouthwestern.edu
>Message-ID: <20061001165553.32345.qmail@web61219.mail.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>PhiHo:
> I am attaching the procedure under separate cover.
> Reni J.
>
>PhiHo Hoang wrote:
>
>Greetings,
>
>I am a new subscriber to this list and I am reading
>the list archive.
>
>I came across a posting from Reni J. responding to
>a request from Sharon:
>
>
>
>Sharon:
>I am sending privately a procedure I have that substitutes xylene
>with
>mineral oil. It is 100 times less toxic than xylene and 3 times
>cheaper.
>Reni J.
>
>Sharon Allen exchange.hsc.mb.ca> wrote:
>
>Does anyone use xylol substitutes, if so how good are they?
>We do CJD testing & "the powers that be" don't like to pay for
>getting rid
>of contaminated xylol. I don't want to have to start testing
>different
>substitutes, so any help would be greatly appreciated.
>Thanks
>Sharon
>sallen <@t> hsc.mb.ca
>
>
>
>I am interested in this procedure.
>
>It is very much appreciated if I can get a copy
>of that procedure.
>
>Best regards,
>
>PhiHo
>
>
>
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>
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>
>
>
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>End of Histonet Digest, Vol 35, Issue 1
>***************************************
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