RE: [Histonet] processing polyester filters

From:"Liz Chlipala"


We have stained mattek cell cultures, we fixed our for 24 hours and then
processed routinely.  We wrote a Sakura Tissue tek article in Histologic you
should be able to see it on line in the archive issues.  Our material and
methods are included.  We have also stained porcine epithial cells that were
seeded into plastic tissue scaffolds and we processed those routinely after
fixation and those also worked just fine.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Perry,
Sent: Wednesday, November 08, 2006 9:38 AM
Subject: [Histonet] processing polyester filters

I am resending this message because we did not receive Vol 36 Issue 6 of
the digest.  We have a researcher who is growing porcine intestinal
epithelial cells on snapwell polyester filters.  He wants us to stain
with the PAS and alcian blue.  Our first attempt did not work.  The
filters were fixed in 4% paraformeldehyde for 10 minutes and processed
in our regular overnight processing schedule. Our first thought is that
we processed way too long.  What type of processing schedule should we
be using and how long should we be fixing these filters?  



Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007


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