The Von Kossa technique stains solid deposits of calcium salts.  While
hypercalcaemia is common in patients undergoing treatment for tuberculosis,
resulting in elevated serum and cytoplasmic calcium levels, this is calcium
in solution, which would not stain with Von Kossa.  I' m not aware of any
kind of intracellular calcium deposits that could be demonstrated with Von
Kossa?

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of Liz
> Chlipala
> Sent: 	Wednesday, November 1, 2006 3:10 PM
> To: 	'Histonet'
> Subject: 	[Histonet] intra cellular calcium
> 
> I know this might be a bit of a silly question, but we have been running a
> lot of von kossa on m. tuberculosis infected guinea pig lungs.  The
> pathologist is telling us that he sees deposistion of calcium that is
> extra
> cellular but not intra cellular and was wondering if the von kossa does
> stain intra cellular calcium.  My general answer to that would be yes, but
> I
> decided to post this question to the Histonet to see if anyone had any
> info
> they were willing to share.
> 
>  
> 
> Thanks in advance
> 
>  
> 
> Liz
> 
>  
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> 
> Manager
> 
> Premier Laboratory, LLC
> 
> P.O. Box 18592
> 
> Boulder, CO 80308
> 
> phone (303) 735-5001
> 
> fax (303) 735-3540
> 
> liz@premierlab.com
> 
> www.premierlab.com
> 
>  
> 
> Ship to Address:
> 
>  
> 
> Premier Laboratory, LLC
> 
> University of Colorado at Boulder
> 
> MCDB, Room A3B40
> 
> Boulder, CO 80309
> 
>  
> 
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