Laying them on a cold (but not below freezing) flat pedestal and snap
freezing them under powdered dry ice should make them lie flat.  How
fast are you getting them frozen?  The OCT may be absorbing cold and
slowing the rate of freezing.  You don't need it.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten@myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey
Barrick
Sent: Thursday, November 16, 2006 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] freezing brain slices

Hello..
   
  I'm trying to freeze brain slices for immuno. The slices are 250uM cut
with a vibratome. I have been trying to embed these slices in OCT so
that I may section them using the cryostat. It seems as though the
tissue is not freezing flat. Does anyone have any suggestions? I cannot
get the slices to freeze flat no matter what i try. 
   
  Thank you!
  Stacey
  barrickstacey@yahoo.com
   

 
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