RE: [Histonet] about methyl green pyronin stain for plasmacytoma...

From:"Kemlo Rogerson"

I amwondering if anyone would tell me the limitations of methyl green
pyronin for plasmacytoma. 

Though I am looking foolish, I think RNAs would increase in the
cytoplasms of most actively proliferating  tumor cells, so I am curious
is it possible to use this stain to differentiate plasmacytoma cells
from other tumor cells based on the pyroninophilia of the cytoplasm. I
remember reading somewhere this stain is non specific, but I could not
find it....please help me, clarify me....on this subject


Methyl Green Pyronin, if used correctly will differentiate between DNA
(green) and RNA (red). It is best after neutral and acid alcoholic
fixatives (Carnoy) as fixation affects staining.


I regard to your question I guess (and I'm not a Histopathologist) that
you would have to have plasmacytoma as a differential in your diagnosis
and the stain used as an aid. I don't think you can use the stain
'litmus' for this type of tumour as that would make too easy. I guess
there are many conditions that result in an increase in the amount of
RNA in a nuclease other than just malignancy. It is differentiates
between DNA and RNA but is not specific (bacteria stain red too) and is
therefore an aid. 


Kemlo Rogerson

Pathology Manager

Ext  3311

DD   01934 647057

Mob 07749 754194

Pager 07659 597107


Habits of thinking need not be forever. One of the most significant
findings in psychology in the last twenty years is that individuals can
choose the way they think. --Martin Seligman 




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