RE: [Histonet] Re: processing possible TB infected tissue
In the veterinary world we are using all dedicated equipment for all prion work. From forceps used to trim tissue to stainers doing the IHC work its all dedicated for prion work only. Twice the equipment, twice the cost.
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
From: email@example.com [mailto:firstname.lastname@example.org] On Behalf Of RSRICHMOND@aol.com
Sent: Thursday, November 09, 2006 12:00 PM
Subject: [Histonet] Re: processing possible TB infected tissue
Jim Vickroy at Memorial Medical Center [where?] asks:
>>We are reviewing our procedures and have a procedure that we really haven't
been following. This is a procedure that calls for processing possible TB
tissue by hand. This was based on an old report that possible TB bacteria could
survive formalin fixation. Right now we are processing all of our tissue by
automated tissue processors, and we really only handle CJD tissue differently
than the others. Obviously we don't have many cases suspected of TB but we do
occasionally process granulomas that are suspicious for AFB positive organisms.
The final diagnosis of these organisms is usually not known until long after
Acid-fast bacilli are killed by formaldehyde fairly quickly, though as always
cutting thin and fixing overnight provides an extra margin of safety. Hand
processing of infected tissue is unnecessary. In at least half of cases a
pathologist sees, the diagnosis of acid-fast disease wasn't suspected before the
slides arrived. I think you should change your procedure.
A question I've never gotten an answer to: is human tissue from AIDS patients
with Mycobacterium avium-intracellulare (MAI) infection a satisfactory
control for Mycobacterium tuberculosis stains?
Prions in Creutzfeldt-Jakob disease and other prion diseases, as is well
known, survive both formaldehyde and paraffin. Ordinary hospital pathology labs
probably shouldn't process prion-infected tissue at all. I think that specialty
labs do process it by hand. What are the veterinary labs' policies for bovine
spongiform encephalopathy (mad cow disease) and chronic wasting disease of
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