[Histonet] x-gal protocol

From:Atoska Gentry

Hello Rob, I'm wondering if you'd be so kind as to share with me your 
x-gal staining procedure ASAP? I have a colleague who's complaining 
about losing the majority of her x-gal staining on frozen brain 
sections. She said the staining looks great until she reaches xylene, 
then a fair amount disappears.  She said that she didn't use a 
counterstain and her supervisor feels a counterstain will aide in better 
viewing of the staining results. I recently came across a reply you made 
to a Beth Roche in a similar situation by way of histonet archives. 
However, your protocol was not shown. Your prompt reply will be much 
appreciated. Atoska

Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras@vetmed.auburn.edu

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