[Histonet] pH of EDTA
We are having a problem with EDTA pH 8.0 from LabVision. When we take
the pH of the concentrate it is 8.O. When we dilute it as recommended
it changes to 6.0. Lab Vision replaced our bottles but the new
bottles also come out to 6.0 when diluted. We took the pH of the
Distilled water here and got 7.1. What could be the problem.? Do we
adjust the distilled water to a higher pH? How do we do that? Do we
then need to validate all of our antibodies that use EDTA using the"
new" EDTA? We have also had this problem with Zymed EDTA but not as
radical a difference as the Labvision. It was suggested that we
switch to ready-made EDTA but we have a quantity of this and do not
have the room to store multiple big bottles of this retrieval buffer.
Can someone give me a suggestion? Thanks, Donna, SRHS
Use your PC to make calls at very low rates
Histonet mailing list
<< Previous Message | Next Message >>