[Histonet] aquaporin 4
Hello Histonetters,
I'm starting immuno for aquaporin 4 with Chemicon anti synthetic peptide =20
(AB3068). After reading some papers describing use of this antibody it
seems to me that it's fairly termolabile. Has anybody working protocol for
this? Any advice is wellcome.
Best regards
Olek Michalski
PS. Since the only secondary ab that fits my needs is produced in donkey =20
and I don't have donkey serum I'm going to use horse serum. My coleague
told me this works for some other stainings. Do you consider it really bad
idea?
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