[Histonet] Will Xylene destroy BDA

From:Niaz Sahibzada

Hi All!
I have injected 10% Biotinylated Dextran Amine (BDA, 10k M.W.) into a CNS
nucleus hoping to see anterograde label in the periphery (i.e., smooth
muscle). Since I need 2µm sections, paraffin embeding is the only way to go.
Does anybody  know if the procedure for deparaffinization will adversely
effect the BDA label (i.e. Destroy the biotin)? Please advice.
Niaz Sahibzada, Ph. D.
Associate Professor
Department of Pharmacology
Rm. #410 NW‹Med-Dent Bldg.
Georgetown University Medical Center
3900 Reservoir Road, NW
Washington, D. C. 20057

Tel: (202) 687-1500
e-mail: sahibzan@georgetown.edu

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