We are having a lot of difficulty finding an inexpensive yet reliable
source for helicobactor pylori control tissue.  We are currently
purchasing them, but often they don't work consistently, and we've
gotten mixed results using slides even from the same box.  In short, we
need a new source.

This is my question:  We have had success culturing all of our other
bacteria; then after we grow a sufficient amount, we inject a liquid
suspension into the lumen of appendix, fix and process the appendix and
use the resulting tissue sections as positive controls (works for pcp,
gram, spirochete, etc.).  However, we can't seem to get h.pylori to grow
the same way.  Is anyone else out there using this technique to make
controls, and if so have you had the same problems growing helico?  And
would it be acceptable to use camphy bacter instead (which I've heard is
more hearty and easy to culture)?-----------------------------------------

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