[Histonet] Toluene and Flouresence Staining
I am studying tracheal vasculature by using vWF as blood vessel marker.
After staining i am using toluene to clear the tissue, but after using that flouresence goes down..................??
Can anyone guide me for to slove this issue.
If some one have a better protocol for tracheal whole mounnt staining, I would love to try for that.
Regard
Dr. Rudiger Tom
University of Connecticut, Storrs
Connecticut
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