[Histonet] RE: Bubbling artifact
We have had something similar happen in our lab in the past. Our pathologists noticed the bubbling on biopsy slides on occasion, on random slides. We turned down the heat on our slide dryers to around 60 C, and we haven't had any problems since. So, were your slides dried together? Over-heating in the dryers can cause bubbling in tissue (I think I've seen that in Frieda Carson's book somewhere). Also, over-fixation can cause nuclear bubbling, but it sounds like that's not the cause in your case.
Tina Mrosla (HTL)
St. Joseph's Hospital
St. Paul MN 55102
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