[Histonet] Help

From:annamaria touvra

  I would appreciate if you could give me some advise on the Von Kossa silver method protocol on frozen sections of aorta. Could you please tell me how could I do the last part of dehydration (use of ethanol) and clearing (use of xylene) of the sections?
  I use for the dehydration 90% ethanol for 1 minute and 100% ethanol for 1 minute, 
  and for the clearing 1 minute Xylene. I think I do not get good and acceptable results, while control samples stain with the same pattern.
  Thank you in advance,

Anna-Maria Touvra MSc
Democritus University of Thrace
Department of Physical Education and Sports Sciences 
Address: Kouloglou 4, Komotini 69100, GREECE
Tel: 00306938511677, 00302531030343

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