Re: [Histonet] weak antibody signal

From:"Jan Shivers"

DAKO sells an LSAB+ kit (labeled streptavidin-biotin-HRP) that is against
mouse, rabbit, AND goat IgGs.  I use it for my goat primary antibodies and
it works well.

Jan Shivers
U of MN Vet Diag Lab

----- Original Message ----- 
From: "Till, Renee" 
Sent: Tuesday, November 22, 2005 10:44 AM
Subject: [Histonet] weak antibody signal

We have a BTEB1 goat polyclonal antibody that is staining very weakly
using a Vectastain kit and DAB. I personally like to order my
secondaries and streptavidin separate, that way I can get F(ab')2
fragements or what have you, if I want. I was going to suggest trying
this, as it is has worked better for me on some stains than the Vector
kits. Is there anything else that could be done to boost the signal? I
had thought of CSA kits and the like, but from what I can tell the Dako
kit at least isn't for goat. Would it work better perhaps using
fluorescence instead of DAB?

Renee' Till, HT

Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR


(501) 364-2774


Confidentiality Notice: This e-mail message, including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential
and privileged information.  Any unauthorized review, use, disclosure or
distribution is prohibited.  If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of the original
Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>