Re: [Histonet] best formalin
We like a neutral buffered formalin (phosphate salts) and purchase it
either ready to use or in concentrated form (to make a final working
solution) We find NBF concentrates convenient and economical. Several of
our labs prefer ready to use phophate buffered formalin i.e.
NBF(Fisherbrand) in order to avoid formaldehyde fumes during NBF
preparation without an available fume hood.
Richard Allan, Surgipath and others have formalin concentrates
available. Surgipath has Carsons Millonigs Buffered Formalin with slightly
different buffering formulation using sodium hydroxide and a phosphate
salt, and MBF also comes in concentrated form. The joy of this one is - it
was developed for use with both FFPE and EM per Freida Carson's publication
in JOH many years ago.
All of the above provided excellent fixation.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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