Re: [Histonet] Osteopontin and GST-a or GST A1-1 Ab for IHC staining on FFPE

From:Dana Settembre

Hello Dusko,
I use osteopontin from Novocastra Laboratories in the UK
catalog # NCL-O-Pontin
I pretreat with a steamer with DakoCytomations Target Retrieval
Solution for 40 minutes
I use the antibody at a dilution of 1:100 for 15 minutes room temp.
Good Luck
Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> "Trajkovic, Dusko"  11/28/2005 1:07:11
PM >>>
Does anyone have a protocol for these antibodies?

I would appreciate any response.

Thank you,

Dusko Trajkovic

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