Re: [Histonet] Microtomy
If one writes an SOP for microtome cleaning, I strongly suggest you find
out the manufacturer recommendations on what they use the job. Removal of
lubricants or not replacing them can create problems - something I learned
At 12:31 PM 12/1/2005, you wrote:
>Although I do not use this kind of microtome in particular, I do dismantle
>and clean my blade holder after every use. I find it needs it and long
>term maintenance as well as section quality is better for my machine. If
>someone who knows what they are doing does the cleaning then there should
>be no problem over time -- to ensure this I completely orient new users to
>every aspect of the machine.
>That being said, this tech should follow whatever the working procedure is
>in your lab. I agree with Rene J in that a written SOP should solve your
>problems. Write one for the lab as a whole or for each brand of microtome
>and insist that the techs follow it.
>At 10:24 AM -0800 12/1/05, Rene J Buesa wrote:
>> First it is not necessary to take appart a knife holder daily. For
>> what you describe
>> this techs acts as some do, thinking that they are like "independent
>> within the lab setting able to do anything the want regardless of
>> general rules.
>> Do you have an SOP that addresses this cleaning procedure? If you
>> don't you
>> can write this aspect and have everybody to adhere to it.
>> With some statistics about recuts required for bad technique you can
>> also try
>> to convince her.
>> You could also get advise from the sales representative regarding this
>> If everything fails you will have to impose authority on the issue.
>> Hope you will be able to reign in this stubborn tech.
>> Rene J.
>>Cindy DuBois wrote:
>> This is a question for those techs who cut on a manual Reichert-Jung 2030.
>>I have one tech who insists on completely taking apart her knife holder
>>everyday to clean it. She removes the springs and all. This person is
>>also the only tech who has trouble cutting and is constantly having to
>>recut a specimen or nearly cutting through needle biopsies.
>>I maintain that handling the knife holder spring so much causes the
>>problems (knife won't clamp tight evenly, chatter and skipping). The
>>other techs, including myself only dismantle and clean the knife holder
>>about once a month. We have had to purchase new spring and bolts for this
>>microtome about every 6 months, whereas the other microtomes have never
>>had to be replaced. How do I go about telling this person to quit
>>cleaning so much.
>>I have had the knife holder and microtome serviced to make sure there is
>>nothing else wrong. I have cut on that machine and find it cuts
>>beautifully as long as you don't take it apart after every 10 blocks to
>>clean behind the blade.
>>Any suggestions would be greatly appreciated,
>>Integrated Pathology Assoc.
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Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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