Re: [Histonet] Dark staining

From:Jennifer MacDonald

We noticed this when someone used the freezing spray too much.  It gives a 
burn affect and very dark staining.

Rene J Buesa  
Sent by:
11/28/2005 09:12 AM

"Webb, Dorothy L" ,

Re: [Histonet] Dark staining

Are the GI biopsies fixed immediately after being taken?
  Do some by any chance air-dry before fixing?
  Are all the sections of the same thickness?
  I would check on these
  Rene J.

"Webb, Dorothy L"  wrote:
  I am wondering what causes some of our tissues to stain with hematoxylin
darker than others? The pathologist is noticing it mostly on GI
biopsies or colon biopsies, not all of them on the same day either. I
can't believe it is due to improper fixation, as all of them are getting
practically the same amount of time in formalin and it is very adequate,
often more than 24 hours. Any ideas would be appreciated. We use
Gills I hematoxylin and Eosin from sigma.
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