Re: [Histonet] Collagen & Elastin IHC on Frozens
Why use expensive immunohistochemical reagents to stain
collagen and elastin? There are plenty of less expensive
ways to do the job using dyes, which are VERY much cheaper
than antibodies. Immunohistochemistry is needed only
for specific identification of particular types of collagen.
You don't say who and where you are. Are you a graduate
student, a technician, a senior professor, a pathology
resident, a captain of industry, or a laboratory mouse
cloned from some of the DNA in Einstein's formadehyde-fixed
brain? You will get more and better replies if you give
more information about what you are trying to investigate.
> Hello Folks,
> I would guess that many of you have done IHC for collagen and elastin on
> frozen sections. Are there any antibodies you would suggest and any tricks for
> using them. I will be working with sheep tissues, primarily lung and
> isolated pulmonary vessels.
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